5KP5
Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.162, 101.162, 106.234 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.610 - 2.100 |
| R-factor | 0.1601 |
| Rwork | 0.159 |
| R-free | 0.18990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ysl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.362 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 87.610 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.112 |
| Number of reflections | 37159 |
| <I/σ(I)> | 15.5 |
| Completeness [%] | 100.0 |
| Redundancy | 19.5 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 6% PEG 8000, 20 mM (NH4)2SO4, 1X MMT pH 6.5 |
