5KO9

Crystal Structure of the SRAP Domain of Human HMCES Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97926
Spacegroup name	I 1 2 1
Unit cell lengths	80.102, 44.745, 82.901
Unit cell angles	90.00, 107.15, 90.00

Refinement procedure

Resolution	48.370 - 1.500
R-factor	0.1831
Rwork	0.182
R-free	0.20930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f20 2bdv 2icu 1zn6
RMSD bond length	0.011
RMSD bond angle	1.499
Data reduction software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.370	48.370	1.530
High resolution limit [Å]	1.500	8.220	1.500
Rmerge	0.045	0.026	0.679
Number of reflections	43639
<I/σ(I)>	15.9	49.8	1.9
Completeness [%]	97.0	98.1	93.2
Redundancy	3.8	3.7	3.4
CC(1/2)	0.999	0.999	0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M BTP, 2% Tacsimate, 20% PEG3350