5KNB
Crystal structure of the 2 ADP-bound V1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-30 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 127.350, 129.620, 237.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.552 - 3.251 |
R-factor | 0.2107 |
Rwork | 0.209 |
R-free | 0.24470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vr4 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.673 |
Data scaling software | XDS |
Phasing software | PHASER (2.5.3) |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.450 |
High resolution limit [Å] | 3.251 | 3.251 |
Rmerge | 1.064 | |
Number of reflections | 62128 | |
<I/σ(I)> | 10.33 | 1.88 |
Completeness [%] | 99.4 | 97.2 |
Redundancy | 6.7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | PEG3350, sodium fluoride, Bis-tris propane |