5KLP

Crystal structure of HopZ1a in complex with IP6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 1 21 1
Unit cell lengths	71.153, 99.777, 71.915
Unit cell angles	90.00, 103.44, 90.00

Refinement procedure

Resolution	49.889 - 2.002
R-factor	0.1697
Rwork	0.168
R-free	0.21360
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.207
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	119760
<I/σ(I)>	17.3
Completeness [%]	98.5
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	277	18-26% PEG 550 MME, 0.1 M sodium citrate, pH 5.2, 200 mM ammonium acetate