5KLI
Rhodobacter sphaeroides bc1 with stigmatellin and antimycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-21 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 118.861, 126.929, 127.891 |
Unit cell angles | 64.64, 87.69, 61.80 |
Refinement procedure
Resolution | 37.744 - 2.996 |
R-factor | 0.2119 |
Rwork | 0.211 |
R-free | 0.24340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qjy |
RMSD bond length | 0.008 |
RMSD bond angle | 1.093 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11rc1_2513: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.070 |
High resolution limit [Å] | 2.996 | 2.996 |
Rmerge | 0.492 | |
Rpim | 0.464 | |
Number of reflections | 112899 | |
<I/σ(I)> | 6.04 | |
Completeness [%] | 92.7 | |
Redundancy | 2.5 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 9% PEG400, 10 mM Sr(NO3)2, 0.06% sucrose mono caprate, 200 mM histidine, 150 mM NaCl, stigmatellin, antimycin |