5KKQ
Homo sapiens CCCTC-binding factor (CTCF) ZnF3-7 and DNA complex structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-05 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.27046 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.994, 44.911, 86.797 |
| Unit cell angles | 98.33, 92.40, 94.80 |
Refinement procedure
| Resolution | 28.936 - 1.744 |
| R-factor | 0.1714 |
| Rwork | 0.170 |
| R-free | 0.19920 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((dev_2257: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.940 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.075 | 0.549 |
| Number of reflections | 110968 | |
| <I/σ(I)> | 12.4 | 1.6 |
| Completeness [%] | 92.6 | 64.1 |
| Redundancy | 3.6 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 25% (w/v) PEG 3350, 0.2 M NaCl, 0.1 M Bis-tris, pH 6.5 |
