5KKI
1.7-Angstrom in situ Mylar structure of hen egg-white lysozyme (HEWL) at 100 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.820, 77.820, 38.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.910 - 1.700 |
R-factor | 0.1661 |
Rwork | 0.161 |
R-free | 0.20970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zix |
RMSD bond length | 0.007 |
RMSD bond angle | 1.026 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.910 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.059 | 0.209 |
Number of reflections | 12515 | |
<I/σ(I)> | 4.28 | 1.38 |
Completeness [%] | 92.0 | 95 |
Redundancy | 1.9 | 1.9 |
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 4.5 | 293 | 100 mM NaOAc, 1 M NaCl, 30% (v/v) PEG 400 |