5KIW
p97 ND1-L198W in complex with VIMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-01 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 153.772, 153.772, 240.641 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.268 - 3.410 |
R-factor | 0.2362 |
Rwork | 0.233 |
R-free | 0.29394 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ko8 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.948 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.410 |
Number of reflections | 22714 |
<I/σ(I)> | 11.6 |
Completeness [%] | 95.9 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 288 | 0.1 M Tris, pH 8, 15 % ethanol, 100 mM NaCl, 7 % MPD |