5KHO

Rasip1 RA domain in complex with Rap1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979460
Spacegroup name	P 21 21 2
Unit cell lengths	101.540, 154.760, 38.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.240 - 2.780
R-factor	0.2088
Rwork	0.205
R-free	0.28570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4HDQ chain B
RMSD bond length	0.009
RMSD bond angle	1.397
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.780	8.200	2.780
Rmerge	0.070	0.027	1.439
Number of reflections	16034
<I/σ(I)>	19.31	55.04	1.45
Completeness [%]	99.5	98.6	98.5
Redundancy	8.3		8.3
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	16% PEG 8K, 200mM Calcium Acetate, and 100mM MES pH 6.5