5KH4
Crystal Structure of Steptococcus pneumoniae Undecaprenyl pyrophosphate Synthase (UPPS) with FARNESYL DIPHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.107, 44.550, 115.542 |
| Unit cell angles | 90.00, 98.69, 90.00 |
Refinement procedure
| Resolution | 19.869 - 3.200 |
| R-factor | 0.2241 |
| Rwork | 0.221 |
| R-free | 0.27600 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.429 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 9847 |
| <I/σ(I)> | 4.7 |
| Completeness [%] | 98.4 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 298 | 0.1M cacodylate, pH 6.4, 0.12-0.24M socium acetate |
