5KH2
Crystal Structure of Steptococcus pneumoniae Undecaprenyl pyrophosphate Synthase (UPPS)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-11-17 |
Detector | MARRESEARCH |
Wavelength(s) | 0.987,1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.687, 118.029, 178.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.21418 |
Rwork | 0.213 |
R-free | 0.24491 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.099 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 53529 |
<I/σ(I)> | 15 |
Completeness [%] | 94.3 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2 ul protein (9.5mg/ml in 50mM Tris-HCl, pH 7.5, 0.2M NaCl, 1mM EDTA) mixed with 2ul reservoir solution containing either 10-20% ethanol, 0.1M Tris-Hcl, pH 8.5 or 4-5% PEG 6000, 0.1M HEPES, pH 7.5 |