5KGL
2.45A resolution structure of Apo independent phosphoglycerate mutase from C. elegans (orthorhombic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.321, 98.852, 173.144 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.437 - 2.450 |
| R-factor | 0.1883 |
| Rwork | 0.185 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ify |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.936 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.17) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 98.850 | 98.850 | 2.540 |
| High resolution limit [Å] | 2.450 | 9.170 | 2.450 |
| Rmerge | 0.205 | 0.051 | 0.840 |
| Rmeas | 0.229 | ||
| Rpim | 0.099 | ||
| Total number of observations | 217179 | ||
| Number of reflections | 43707 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 97.5 | 90.8 | 99.4 |
| Redundancy | 5 | 5.2 | 4.7 |
| CC(1/2) | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25% (w/v) PEG 3350, 0.1 M Bis-Tris, 0.2 M ammonium sulfate |
