5KFI
Human DNA polymerase eta-DNA ternary complex: reaction with 10 mM Mn2+ for 120s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 98.530, 98.530, 81.840 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.896 - 1.650 |
R-factor | 0.1914 |
Rwork | 0.190 |
R-free | 0.22080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.896 | 1.690 | |
High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
Rmerge | 0.070 | 0.019 | 0.702 |
Rmeas | 0.076 | 0.021 | 0.759 |
Total number of observations | 355355 | ||
Number of reflections | 53596 | 604 | 4020 |
<I/σ(I)> | 19.96 | 77.18 | 2.65 |
Completeness [%] | 98.7 | 94.7 | 100 |
CC(1/2) | 0.999 | 0.999 | 0.807 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 298 | 17% PEG 2000MME, 0.1 M MES |