5KFH

Human DNA polymerase eta-DNA ternary complex: reaction with 10 mM Mn2+ for 90s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.350, 98.350, 81.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.860 - 1.720
R-factor	0.1732
Rwork	0.170
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		1.760
High resolution limit [Å]	1.720	7.690	1.720
Rmerge	0.055	0.018	0.731
Number of reflections	47569
<I/σ(I)>	19.94	74.19	2.1
Completeness [%]	99.7	93.6	99.9
Redundancy	4.6
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	17% PEG 2000MME, 0.1 M MES