5KEA
mouse Klf4 ZnF1-3 (E446D) and CpG/CpG sequence DNA complex structure: Form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-11 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 50.850, 50.850, 131.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.406 - 2.458 |
R-factor | 0.2346 |
Rwork | 0.229 |
R-free | 0.28280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m9e |
RMSD bond length | 0.020 |
RMSD bond angle | 0.540 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2400: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.700 | 2.520 |
High resolution limit [Å] | 2.450 | 2.460 |
Rmerge | 0.054 | |
Number of reflections | 6808 | |
<I/σ(I)> | 20.37 | 3.03 |
Completeness [%] | 98.0 | 7.3 |
Redundancy | 7.1 | 3.1 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris-HCl pH8.5 , 0.25M NaCl and 20% polyethylene glycol 8000 |