5KE9

mouse Klf4 E446P ZnF1-3 and TpG/CpA sequence DNA complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-04
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	51.440, 51.440, 130.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.235 - 2.336
R-factor	0.217
Rwork	0.214
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m9e
RMSD bond length	0.005
RMSD bond angle	0.561
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2400: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.235	2.520
High resolution limit [Å]	2.336	2.420
Rmerge	0.099	0.887
Number of reflections	7979
<I/σ(I)>	16.6	4.06
Completeness [%]	99.5	100
Redundancy	10.7	10
CC(1/2)	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	0.1M Tris-HCl pH8.5 , 0.25M NaCl and 20% polyethylene glycol 8000