Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

5KE6

mouse Klf4 ZnF1-3 and TpG/CpA sequence DNA complex structure

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-BM
Synchrotron siteAPS
Beamline22-BM
Temperature [K]100
Detector technologyCCD
Collection date2014-03-08
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)1.000
Spacegroup nameP 43 21 2
Unit cell lengths50.870, 50.870, 130.540
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution34.678 - 1.990
R-factor0.1997
Rwork0.198
R-free0.23370
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4m9e
RMSD bond length0.008
RMSD bond angle0.874
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX ((dev_2400: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]34.6782.040
High resolution limit [Å]1.9901.990
Rmerge0.0730.982
Number of reflections12435
<I/σ(I)>16.582.32
Completeness [%]99.9
Redundancy77.2
CC(1/2)0.906
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.52890.1M Tris-HCl pH8.5 , 0.25M NaCl and 20% polyethylene glycol 8000

227111

PDB entries from 2024-11-06

PDB statisticsPDBj update infoContact PDBjnumon