5KE6
mouse Klf4 ZnF1-3 and TpG/CpA sequence DNA complex structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 50.870, 50.870, 130.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.678 - 1.990 |
R-factor | 0.1997 |
Rwork | 0.198 |
R-free | 0.23370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m9e |
RMSD bond length | 0.008 |
RMSD bond angle | 0.874 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2400: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.678 | 2.040 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.073 | 0.982 |
Number of reflections | 12435 | |
<I/σ(I)> | 16.58 | 2.32 |
Completeness [%] | 99.9 | |
Redundancy | 7 | 7.2 |
CC(1/2) | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris-HCl pH8.5 , 0.25M NaCl and 20% polyethylene glycol 8000 |