5KDV
IMPa metallopeptidase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.280, 96.410, 100.920 |
| Unit cell angles | 90.00, 124.66, 90.00 |
Refinement procedure
| Resolution | 48.720 - 1.930 |
| R-factor | 0.17314 |
| Rwork | 0.172 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.530 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.720 | 2.030 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.088 | 0.489 |
| Number of reflections | 90627 | |
| <I/σ(I)> | 15 | 4.2 |
| Completeness [%] | 98.8 | 97.9 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | PEG 3350, (NH4)2SO4, Bicine pH 9.0 |
