5KD1
Sperm whale myoglobin H64A with nitrosoamphetamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.500, 90.500, 45.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.360 - 1.700 |
| R-factor | 0.149 |
| Rwork | 0.148 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mbw |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.650 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.048 | 0.191 |
| Number of reflections | 23468 | |
| <I/σ(I)> | 23.2 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 5.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 296 | 100 mM Tris-HCl, 1 mM EDTA, pH 9 2.3 M Ammonium Sulfate |
