5KCX
Pim-1 kinase in Complex with a Selective N-substituted 7-azaindole Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2008-11-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 |
Unit cell lengths | 98.010, 98.010, 80.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.000 - 2.200 |
Rwork | 0.190 |
R-free | 0.21120 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNX |
Refinement software | CNX (2005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 21552 |
<I/σ(I)> | 14.7 |
Completeness [%] | 96.0 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | protein stock: 6.26 mg/mL in Tris 20mM - DTT 5mM - NaCl 150mM - 0.5 mM inhibitor - pH8 crystallization solution: imidazole 0.1M - Na Acetate 0.6M - pH6.5 1:1 protein to solution ratio for hanging drop freezing conditions: imidazole 0.1M - Na Acetate 1M - glycerol 20% - pH6.5 |