5KBX
Co-crystal structure of the Saccharomyces cerevisiae histidine phosphotransfer signaling protein Ypd1 and the receiver domain of its downstream response regulator Ssk1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97910, 0.97930, 0.9116 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.696, 71.696, 176.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.270 - 2.800 |
R-factor | 0.2325 |
Rwork | 0.228 |
R-free | 0.27850 |
Structure solution method | MAD |
RMSD bond length | 0.001 |
RMSD bond angle | 0.350 |
Data reduction software | HKL-2000 (708) |
Data scaling software | HKL-2000 (708) |
Phasing software | AutoSol |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.700 | 2.900 |
High resolution limit [Å] | 2.798 | 2.800 |
Rmerge | 0.037 | 0.168 |
Number of reflections | 10695 | 1163 |
<I/σ(I)> | 34 | 9.2 |
Completeness [%] | 89.0 | 44 |
Redundancy | 1.9 | 1.8 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 296 | 0.2 M lithium sulfate, 0.1 M CAPS/NaOH pH 10.5, 1.2 M NaH2PO4/0.8 M K2HPO4 |