5KAR
Murine acid sphingomyelinase-like phosphodiesterase 3b (SMPDL3B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 50.892, 47.031, 93.861 |
| Unit cell angles | 90.00, 98.07, 90.00 |
Refinement procedure
| Resolution | 36.833 - 1.142 |
| R-factor | 0.1355 |
| Rwork | 0.135 |
| R-free | 0.15180 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.833 |
| High resolution limit [Å] | 1.140 |
| Number of reflections | 147638 |
| <I/σ(I)> | 17.4 |
| Completeness [%] | 92.9 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG 3350, ammonium nitrate |
