5KAL
Terminal uridylyl transferase 4 from Trypanosoma brucei with bound UTP and UpU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.688, 42.876, 109.618 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 47.129 - 2.750 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ikf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.566 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev-2447_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.130 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.104 | 0.374 |
| Number of reflections | 20369 | |
| <I/σ(I)> | 7.1 | 2.2 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.52 | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100mM sodium cacodylate pH 6.5, 200mM calcium acetate, 18% PEG-8000, 5mM UTP, 5mM UpU |
