5K9S

Importin-alpha in complex with HNF1-beta peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97624
Spacegroup name	C 2 2 21
Unit cell lengths	81.380, 111.394, 129.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.712 - 2.400
R-factor	0.1872
Rwork	0.185
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u54
RMSD bond length	0.008
RMSD bond angle	0.797
Data reduction software	xia2
Data scaling software	Aimless (0.5.16)
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.400	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	23388
<I/σ(I)>	10.2	8
Completeness [%]	100.0	100
Redundancy	7.4	7.6
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	10% PEG 8K, 0.09 M NPS, 20% ethylene glycol, 0.1 M Buffer 1 (Morpheus I)