5K9Q

Crystal structure of multidonor HV1-18-class broadly neutralizing Influenza A antibody 16.a.26 in complex with A/Hong Kong/1-4-MA21-1/1968 (H3N2) Hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	121.808, 233.909, 302.452
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.277 - 2.503
R-factor	0.1838
Rwork	0.182
R-free	0.21800
RMSD bond length	0.003
RMSD bond angle	0.635
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	244485
<I/σ(I)>	14.05
Completeness [%]	82.5
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.01M L-cysteine, 0.09M sodium cacodylate, pH 6.5, 0.18M magnesium acetate, and 7.9% (w/v) PEG-8000