Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97670 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 92.707, 100.557, 114.017 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.160 - 1.900 |
R-factor | 0.17537 |
Rwork | 0.173 |
R-free | 0.22079 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3D1T |
RMSD bond length | 0.020 |
RMSD bond angle | 1.992 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.160 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.047 | 0.355 |
Number of reflections | 41832 | |
<I/σ(I)> | 36.17 | 2.05 |
Completeness [%] | 99.2 | |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | (PEG 6000, 10-16%), LiCl (1-1.4 M), Tris (50 mM, pH 9.0) and Tris-Cl (50 mM, pH 7.0). Enzym sample prepared at 10 mg/mL in 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, pH 8.0 |