5K8W
Crystal structure of mouse CARM1 in complex with inhibitor U2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.919, 98.817, 207.466 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.925 - 2.100 |
R-factor | 0.1855 |
Rwork | 0.183 |
R-free | 0.24020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ih3 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.586 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2386: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.309 | |
Number of reflections | 90553 | |
<I/σ(I)> | 4.9 | 1.6 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.9 | 5.1 |
CC(1/2) | 0.983 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Tris-HCl pH 8.0 100 mM, PEG 2000 MME 15 % |