5K8T
Crystal structure of ZIKV NS3 helicase in complex with GTP-gammar S and an magnesium ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.950, 53.420, 65.390 |
| Unit cell angles | 90.00, 105.04, 90.00 |
Refinement procedure
| Resolution | 40.403 - 1.848 |
| R-factor | 0.1883 |
| Rwork | 0.185 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wzq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 34574 | |
| <I/σ(I)> | 14.98 | |
| Completeness [%] | 93.9 | |
| Redundancy | 2.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG 6000, 5% MPD, 0.1M HEPES pH7.5 |
