5K70
Sidekick-2 immunoglobulin domains 1-4 H18R/N22S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 110.890, 113.430, 185.906 |
| Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
| Resolution | 39.516 - 2.700 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Sdk1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.064 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.101 | 0.546 |
| Number of reflections | 61442 | |
| <I/σ(I)> | 10.1 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 295 | 13.5% PEG3350 (w/v), 0.1M ammonium sulfate, 0.2M sodium dihydrogen phosphate |
