5K6I

Crystal structure of prefusion-stabilized RSV F single-chain 9-10 DS-Cav1 A149C-Y458C, S46G-E92D-S215P-K465Q variant.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 41 3 2
Unit cell lengths	169.561, 169.561, 169.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.030 - 2.920
R-factor	0.221
Rwork	0.220
R-free	0.25300
RMSD bond length	0.002
RMSD bond angle	0.441
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.028	3.020
High resolution limit [Å]	2.918	2.920
Rmerge	0.140
Number of reflections	18371
<I/σ(I)>	11.57	2.25
Completeness [%]	98.0	94.2
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Na Cacodylate, pH 6.5, 0.2M ZnAC, 17% (w/v) PEG 8,000