5K6C
Crystal structure of prefusion-stabilized RSV F single-chain 9-10 DS-Cav1 variant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 171.221, 171.221, 171.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.427 - 3.576 |
| R-factor | 0.2287 |
| Rwork | 0.227 |
| R-free | 0.26210 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.454 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.710 |
| High resolution limit [Å] | 3.580 | 3.580 |
| Number of reflections | 9782 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 91.5 | 91.2 |
| Redundancy | 5.9 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 0.19 M (NH4)2SO4, 11 % iso-propanol, 17 % PEG 8,000 |
