5K56
Human muscle fructose-1,6-bisphosphatase in active R-state in complex with fructose-1,6-bisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-22 |
| Detector | RAYONIX SX-165mm |
| Wavelength(s) | 0.89430 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 72.581, 72.581, 232.442 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.641 - 2.198 |
| R-factor | 0.2099 |
| Rwork | 0.205 |
| R-free | 0.27980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5et5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.138 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.650 | 2.330 |
| High resolution limit [Å] | 2.198 | 2.198 |
| Rmerge | 0.066 | 0.785 |
| Number of reflections | 16183 | |
| <I/σ(I)> | 28.03 | 3.55 |
| Completeness [%] | 98.8 | 95.4 |
| Redundancy | 9.58 | 9.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | 10mM magnesium chloride, 2M sodium chloride, 10% PEG6000, 10mM Tris |
