5K3J
Crystals structure of Acyl-CoA oxidase-2 in Caenorhabditis elegans bound with FAD, ascaroside-CoA, and ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.040, 85.571, 106.899 |
Unit cell angles | 90.00, 91.43, 90.00 |
Refinement procedure
Resolution | 39.720 - 2.680 |
R-factor | 0.2149 |
Rwork | 0.213 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Acox1a |
RMSD bond length | 0.004 |
RMSD bond angle | 0.931 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.720 | 2.800 |
High resolution limit [Å] | 2.680 | 2.680 |
Rmerge | 0.160 | 0.370 |
Number of reflections | 38639 | |
<I/σ(I)> | 10.9 | 4.6 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 298 | 0.2 M ammonium acetate, 0.1 M Tris-HCl pH 8.5 and 25% w/v PEG 3350 |