5K3H
Crystals structure of Acyl-CoA oxidase-1 in Caenorhabditis elegans, Apo form-II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 153.075, 140.795, 154.930 |
Unit cell angles | 90.00, 117.20, 90.00 |
Refinement procedure
Resolution | 49.241 - 2.480 |
R-factor | 0.1932 |
Rwork | 0.191 |
R-free | 0.23150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1is2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.045 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.241 | 2.520 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.120 | 0.520 |
Number of reflections | 206114 | |
<I/σ(I)> | 14.4 | 0.66 |
Completeness [%] | 99.6 | 93 |
Redundancy | 5.1 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.4 M NaCl, 0.1 M HEPES pH 7.4, 18% w/v PEG 8000 and 8% v/v glycerol |