5K1B
Crystal structure of the UAF1/USP12 complex in F222 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.283 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 120.958, 158.929, 234.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 3.300 |
R-factor | 0.23737 |
Rwork | 0.235 |
R-free | 0.28551 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.547 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.102 | |
Number of reflections | 16052 | |
<I/σ(I)> | 28.2 | |
Completeness [%] | 98.1 | 96.4 |
Redundancy | 8.4 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 0.1 M imidazole, 18% PEG 2000MME, 0.3 M ammonium citrate |