5K0W
Crystal structure of the metallo-beta-lactamase GOB-18 from Elizabethkingia meningoseptica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.000, 48.510, 88.940 |
| Unit cell angles | 90.00, 100.10, 90.00 |
Refinement procedure
| Resolution | 24.330 - 2.610 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k07 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.330 |
| High resolution limit [Å] | 2.610 |
| Rmerge | 0.122 |
| Number of reflections | 16108 |
| <I/σ(I)> | 9.5 |
| Completeness [%] | 96.9 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2M NaCl, 2M (NH4)2SO4, 0.1M Tris.HCl |
