5K0M
Targeting the PRC2 complex through a novel protein-protein interaction inhibitor of EED
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.843, 84.911, 91.627 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.912 - 1.830 |
| R-factor | 0.13598 |
| Rwork | 0.133 |
| R-free | 0.18475 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jzn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.920 | 1.834 |
| High resolution limit [Å] | 1.828 | 1.828 |
| Rmerge | 0.141 | 1.145 |
| Number of reflections | 40632 | |
| <I/σ(I)> | 12.7 | 2 |
| Completeness [%] | 100.0 | 98.6 |
| Redundancy | 6.4 | 6.4 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 290 | 3.08M Sodium Formate, 20% Glycerol, 0.1M Tris pH8.5 |
