5JYE
Structures of Streptococcus agalactiae GBS GAPDH in different enzymatic states
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.247, 107.358, 87.904 |
Unit cell angles | 90.00, 113.01, 90.00 |
Refinement procedure
Resolution | 80.910 - 2.230 |
R-factor | 0.20948 |
Rwork | 0.207 |
R-free | 0.24870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4QX6_A |
RMSD bond length | 0.009 |
RMSD bond angle | 1.369 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.360 | 2.290 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.040 | 0.800 |
Number of reflections | 64548 | |
<I/σ(I)> | 13.4 | 1.1 |
Completeness [%] | 99.1 | 98.8 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20-28% PEG4000, 0.1M Mes |