5JY6
Structures of Streptococcus agalactiae GBS GAPDH in different enzymatic states
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.578, 107.465, 89.273 |
Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
Resolution | 86.600 - 2.000 |
R-factor | 0.19597 |
Rwork | 0.195 |
R-free | 0.21625 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4QX6_A |
RMSD bond length | 0.008 |
RMSD bond angle | 1.360 |
Data reduction software | XDS (March 30, 2013) |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.600 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.084 | 0.467 |
Number of reflections | 82890 | |
<I/σ(I)> | 11.3 | 1.9 |
Completeness [%] | 99.3 | 99.1 |
Redundancy | 2.5 | 2.5 |
CC(1/2) | 0.987 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20-28% PEG4000, 0.1M Mes |