5JX0
Temperature sensitive D4 mutant L110F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-09-11 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.346, 102.916, 116.587 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.080 - 2.400 |
R-factor | 0.22901 |
Rwork | 0.228 |
R-free | 0.25332 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4DOF_A |
RMSD bond length | 0.011 |
RMSD bond angle | 1.476 |
Data reduction software | XDS (March 30, 2013) |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.080 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.054 | 0.658 |
Number of reflections | 44146 | |
<I/σ(I)> | 14.8 | 1.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.4 | 5.4 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 8% PEG8000, 5% Glycerol, 0.1M Tris |