5JWY
Structure of lipid phosphate phosphatase PgpB complex with PE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.533, 73.593, 132.095 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 132.100 - 3.200 |
R-factor | 0.2691 |
Rwork | 0.267 |
R-free | 0.30510 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.276 |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 132.100 | 73.593 | 3.370 |
High resolution limit [Å] | 3.200 | 10.120 | 3.200 |
Rmerge | 0.019 | 0.778 | |
Number of reflections | 6838 | ||
<I/σ(I)> | 25.2 | 31.4 | 1 |
Completeness [%] | 100.0 | 98.9 | 100 |
Redundancy | 14 | 11.2 | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG6000, sodium chloride, ADA |