5JWY

Structure of lipid phosphate phosphatase PgpB complex with PE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	39.533, 73.593, 132.095
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	132.100 - 3.200
R-factor	0.2691
Rwork	0.267
R-free	0.30510
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.276
Data scaling software	SCALA (3.3.16)
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	132.100	73.593	3.370
High resolution limit [Å]	3.200	10.120	3.200
Rmerge		0.019	0.778
Number of reflections	6838
<I/σ(I)>	25.2	31.4	1
Completeness [%]	100.0	98.9	100
Redundancy	14	11.2	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	PEG6000, sodium chloride, ADA