5JWH
Apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2016-03-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.290, 52.600, 65.110 |
| Unit cell angles | 90.00, 105.09, 90.00 |
Refinement procedure
| Resolution | 31.433 - 1.400 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wzq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.963 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 157613 | |
| <I/σ(I)> | 15.92 | 1.81 |
| Completeness [%] | 98.8 | 94.8 |
| Redundancy | 6.89 | 6.07 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG 8000, 5% MPD, 0.1 M HEPES pH 7.5 |
