5JVG

The large ribosomal subunit from Deinococcus radiodurans in complex with avilamycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	I 2 2 2
Unit cell lengths	170.034, 412.632, 698.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.549 - 3.428
R-factor	0.2135
Rwork	0.211
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zjr
RMSD bond length	0.005
RMSD bond angle	1.014
Data scaling software	HKL-2000
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.410
High resolution limit [Å]	3.350	9.080	3.350
Rmerge	0.168	0.052	0.886
Total number of observations	1115549
Number of reflections	290370
<I/σ(I)>	4.7
Completeness [%]	93.3	85.3	89.2
Redundancy	3.8	4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293.15	Ribosome solution containing 0.0065 mM (180 A/ml) of D50S in 10 mM Hepes pH=7.8, 15 mM MgCl2 and 75 mM NH4Cl crystallization buffer was mixed with 10 mM spermidine, 1 % ethanol and 0.5 % 2-ethyl-1,3-hexanediol precipitants. A 0.005 ml crystallization drop was hanged over 10 % ethanol and 5% 2-ethyl-1,3-hexanediol in ddw reservoir.