5JV5
Trypanosome brucei Hypoxanthine-guanine phosphoribosyltranferase in complex with Guanosine 5' monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-18 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.95369 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.944, 111.711, 45.226 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.011 - 2.730 |
R-factor | 0.2399 |
Rwork | 0.236 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jsq |
RMSD bond length | 0.002 |
RMSD bond angle | 0.558 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASES |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.010 | 2.860 |
High resolution limit [Å] | 2.730 | 2.730 |
Rmerge | 0.160 | 0.760 |
Number of reflections | 13262 | |
<I/σ(I)> | 8.6 | 1.9 |
Completeness [%] | 99.9 | 99.2 |
Redundancy | 7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 25% PEG 3350, 0.2 M lithium sulfate and 0.1 M Bis-Tris |