5JU5
Crystal structure of the human Tankyrase 1 (TNKS) SAM domain (D1055R), crystal form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.237, 55.222, 83.046 |
Unit cell angles | 90.00, 96.16, 90.00 |
Refinement procedure
Resolution | 82.570 - 2.500 |
R-factor | 0.192 |
Rwork | 0.191 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Homology model based on 5JRT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.570 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 16507 | |
<I/σ(I)> | 12 | |
Completeness [%] | 100.0 | |
Redundancy | 27.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285.15 | 0.1 M Bis-Tris pH 5.5 0.2 M Magnesium chloride PEG 3350 25% |