5JTC
Aspartyl/Asparaginyl beta-hydroxylase (AspH)oxygenase and TPR domains in complex with manganese, 2,4-pyridine dicarboxylate and factor X substrate peptide fragment(39mer-4Ser)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.020, 91.660, 123.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.508 - 2.240 |
R-factor | 0.1826 |
Rwork | 0.181 |
R-free | 0.20850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jqy |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2386) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.660 | 2.300 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.103 | 0.895 |
Number of reflections | 28029 | |
<I/σ(I)> | 19.8 | 3.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.1 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 20% PEG3350, 200mM NaBr, 100mM BisTris Propane, 1mM MnCl2, 2mM 2,4-PDCA, 330uM ASPH, 726uM Factor X |