5JTA
Neutral trehalase Nth1 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 186.700, 186.700, 119.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.150 - 2.720 |
| R-factor | 0.21345 |
| Rwork | 0.211 |
| R-free | 0.26040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Preliminary model obtained from SAD experiment |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.160 | 2.880 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmeas | 0.187 | 1.290 |
| Number of reflections | 33613 | |
| <I/σ(I)> | 14.09 | 2.04 |
| Completeness [%] | 99.9 | 99.99 |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.3 | 291 | Sodium Citrate, Ammonium Sulfate, Lithium Sulfate |
