5JT8
Structural basis for the limited antibody cross reactivity between the mite allergens Blo t 1 and Der p 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-05-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.933 |
| Spacegroup name | H 3 |
| Unit cell lengths | 117.412, 117.412, 132.364 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.468 - 2.100 |
| R-factor | 0.1728 |
| Rwork | 0.170 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xkg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.029 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 55.486 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 79280 |
| <I/σ(I)> | 28.8 |
| Completeness [%] | 100.0 |
| Redundancy | 17.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | single crystals were obtained with drops containing 4 ul sample solution containing 1 mg/ml rproBlo t 1, 0.5 mM YCl3, and 10 mM Tris-HCl pH 8.0, and 2 ul reservoir solution containing 4% w/v PEG-6000, 0.1 M Na-HEPES pH 6.6 and 0.8 M LiNO3 |
