5JSG
Crystal structure of Spindlin1 bound to compound EML405
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.060, 123.908, 49.779 |
| Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
| Resolution | 38.786 - 2.500 |
| R-factor | 0.192 |
| Rwork | 0.186 |
| R-free | 0.24490 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.538 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10_2155: ???) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 6.160 | 2.500 |
| Rmerge | 0.135 | 0.049 | 0.834 |
| Total number of observations | 64262 | ||
| Number of reflections | 18249 | ||
| <I/σ(I)> | 10.2 | ||
| Completeness [%] | 99.7 | 98.6 | 99.7 |
| Redundancy | 3.5 | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2M MgCl2, 0.1M HepesNa, 20% PEG 10000 |
