5JSA

Uncleaved prefusion optimized gp140 trimer with an engineered 10-residue HR1 turn bound to broadly neutralizing antibodies 8ANC195 and PGT128

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	I 2 3
Unit cell lengths	266.279, 266.279, 266.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.143 - 6.308
R-factor	0.285
Rwork	0.281
R-free	0.32180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c7k
RMSD bond length	0.004
RMSD bond angle	0.841
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	6.520
High resolution limit [Å]	6.300	6.300
Number of reflections	6914
<I/σ(I)>	17.9
Completeness [%]	100.0	100
Redundancy	15.1
CC(1/2)	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	0.05 M lithium sulfate, 0.05 M sodium sulfate, 20% w/v PEG400, 0.05 M Tris-HCl, pH 8.7